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The plasmids were transformed into E

GCK also exerts joint protection by inhibiting the proliferation, migration and secretion of synovial cells (129), whose molecular mechanism is to inhibit the secretion of TNF- in AIA-FLS rats, down-regulate the expression of tumor necrosis factor receptor type 2 (TNFR2), and inhibit TNF--mediated proliferation, migration and secretion of AIA -FLS

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After washing with PBS, samples were incubated with Alexa Fluor 488- or 555-conjugated secondary antibodies (Invitrogen, California, A-21202/A-31572, USA) for 1 h at room temperature in the dark and stained with 4,6-diamidino-2-phenylindole (DAPI)

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