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Laboratory testing can also determine if DNA adducts are present

After washing with TBST (50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween-20 and pH 7.6), the fixed cells were blocked using Blocking One reagent (Nacalai Tesque) containing 0.1% Triton X-100 for 1 h at 25 C

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Pharmaceuticals, 2025

A partial cDNA of AsGGT , which has high sequence homology to AcGGT , was isolated from garlic ( Allium sativum ), and its mRNA expression patterns suggested that AsGGT may play a role in synthesizing S -alk(en)yl-L-cysteine sulfoxides in garlic cloves during cold storage ( In this study, we cloned three genes encoding GGTs, AsGGT1 , AsGGT2 , and AsGGT3 , that are suggested to be involved in the biosynthesis of alliin in garlic