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For the AAV, AAV-TBG- Chac1 was generated from AAV-TBG-GFP (Addgene, 105535) by replacing the GFP sequence with Chac1 -myc subcloned from pcDNA3.1- Chac1 -myc

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After the initial wave of Harding lab publications in 2012 and 2013, academic publication on Dihexa specifically has slowed considerably