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Clicks media (Sigma-Aldrich) was supplemented with 10% FBS (Biological Industries), L-glutamine (2 mM), penicillin (100 U/mL, Biological Industries), streptomycin (0.1 mg/mL, Biological Industries) and -mercaptoethanol (40 nM, Biological Industries)

Water only changes the concentration and the volume, never the total milligrams