Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer
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Agriculture Trading AG, Walenstadt, Switzerland), at 1500 mol m 2 s 1 for 4 h, which resulted in the accumulation of H 2 O 2 in the cytosol and along the tonoplast in vacuoles, increased density of immunolabeled Asc and total glutathione (= sum of GSH and GSSG) in chloroplasts, peroxisomes and the cytosol, degradation of thylakoid membranes (plastoglobuli formation) and an overall increase in H 2 O 2 level and CAT activity in the leaves after 14 days of treatment
Idal pour les peaux sches mixtes en qute dclat, il offre une texture riche mais fondante qui pntre rapidement sans effet collant, laissant la peau douce et lumineuse
doi: 10.1084/jem.20221847 60 LesliePLChaoYLTsaiYHGhoshSKPorrelloAVan SwearingenAEDet al
