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Plates were then incubated overnight at 4 C with either primary phospho-(p)NRF2 antibodies (Abcam, Cambridge, UK, phospho-Ser40, Rabbit monoclonal antibody, Cat# ab76026, RRID:AB_1524049, dilution 1:500) Nrf2 antibodies (Abcam, Rabbit monoclonal antibody, Cat# ab62352, dilution 1:200), or Ki67 antibodies (Abcam, Rabbit polyclonal antibody, Cat# ab15580, dilution 1:400) in 2% NGS in 1 PBS

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This is done because the vial is a vacuum, and you need to add an equal amount of air to regulate the pressure