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illustrated that the absence of eosinophils led to an increase of Nos2 + Arg1 + macrophages and Ifng + Th1 cells as well as to a decrease of Mrc1 + Cd163 + macrophages and M2-like macrophages (Fig

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Northern analysis RNA was isolated using TRI-Reagent (Sigma-Aldrich)

Similarly, western blotting analysis confirmed that the expression of GPX4 in LA treated-mice was upregulated in a dose-dependent manner, compared with downregulation in FA injected-mice, although there was no significant statistical difference between two dose groups

Many osmolytes do not directly interact with proteins but exhibit osmoprotectant activity by excluding them from the protein surface (i.e., osmophobic effect), allowing proteins to fold correctly (Diamant et al., 2001