When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr
Improved Blood Circulation Healthy blood circulation is essential for strong and consistent beard growth
However, the use of blinded evaluators (dermatologists who didn't know which photos were "before" and "after") and AI-assisted assessment helps reduce subjective bias in the results
doi:10.4103/jrms.JRMS_862_18 De Bruyn E, Gulbis B, Cotton F
Conventional treatments range from rest and ice to custom orthotics, physical therapy, night splints, extracorporeal shock wave therapy, and corticosteroid injections