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doi: 10.1016/j.molmed.2021.06.002 202 ErcoliGFernandesVEChungWYWanfordJJThomsonSBaylissCDet al

The 10mg/mL concentration works well for this goal: 4mg requires 40 units and 8mg requires 80 units, both clean readings on a standard syringe

Following the reverse transcription, qPCR was performed to quantify the RNA levels of NLRP3, ASC and caspase-1 using SYBR-Green Supermix (Bio-Rad, Hercules, CA, USA) and the data were analyzed using the 2Ct method

L., Chin, L., Wagner, G., Asara, J