2 Subordinate Classification According to statistics, there are over 300 cultivated varieties of durian worldwide
The transfection mixture was added drop-by-drop to wells and incubated for 24 h
The samples were incubated for 1 h at RT, washed three times with TBST, reacted with Supersignal West Pico solution (Pierce) for 5 min, and exposed to X-ray film (Agfa)
Mass spectrometric analysis and detection were performed using specific MRM for analytes and their related labeled internal standards, on a Xevo TQ-XS (Waters , United States), equipped with electrospray ionization source in positive mode and argon as collision gas
Immunohistochemical staining for S100, SOX10, HMB-45, Melan-A, p16 and vimentin yields positive results, whereas GFAP, EMA, and CK staining are mostly negative (4145, 49, 50)