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Microvascular dysfunction in blood-brain barrier disruption and hypoperfusion within the infarct posttreatment are associated with cerebral edema

To uncover the underlying mechanisms, the researchers analyzed hippocampal tissue focusing on dentate gyrus, where they quantified neural progenitor proliferation and neurogenesis through BrdU, immunofluorescence for Sox2, GFAP, DCX and NeuN, and unbiased stereological cell counting

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After the glycosylation, the affinity support, the protein is bound to unbound contaminants are washed away, and the Eluted protein was purified