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In summary, this experimental design combined pharmacological treatments, CRISPR-mediated receptor knockout, functional motility assays, protein-level detection, and transcript quantification to investigate the effects of Gepon and TGF-1 in fibroblasts
doi:10.3390/ph17040461 McGuire FP, Martinez R, Lenz A, Skinner L, Cushman DM
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All qRT-PCR samples were run in technical duplicates and the Ct method based on the reference value estimate from 3 housekeeping gene (GAPDH, GUSB and TBP) was used to calculate relative expression level of each gene and reaction