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Since a 12 amino acid difference is difficult to resolve by SDS-PAGE, the 70-residue Propionibacterium shermanii transcarboxylase domain (PSTCD) is fused on the N-terminus of L2 to generate a 9 kDa size shift upon furin cleavage

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Down this line, we observed that FA administration induced a transient decrease in FPN expression, while LA supplementation significantly upregulated FPN levels, thereby accelerating mobilization of excessive iron out of tubular cells and reducing intracellular iron overload

Wilson WA, Hughes WE, Tomamichel W, Roach PJ

2.3 MTS assay GDC was dissolved in DMEM D1145 and filter sterilized using 0.22 m PET syringe filters, (A16532-GUK, Misart Engineering Ltd, New Zealand), to a concentration of 0.5 to 5 mM