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The ubiquitin-proteasome system in kidney physiology and disease
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To remove cell debris, the plates were centrifuged for 10 min at 3500 RPM and 4 C, before the supernatants were collected
Two key enzymes, acyl-CoA synthetase long-chain family member 4 (ACSL4) and lysophosphatidylcholine acyltransferase 3 (LPCAT3), are involved in the biosynthesis and remodeling of PEs, activation of PUFAs, and insertion of PUFAs into membrane PLs (Kagan et al., 2017), while LPCAT3 preferentially catalyzes the insertion of acylated AA into membrane phospholipids (Kagan et al., 2017)