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On the next day, sections were incubated with Polymer Helper-conjugated secondary antibodies for 20 min in a 37C incubator and then rinsed with PBS three times for 5 min each

These PCs significantly lowered fasting blood glucose, improved glucose/insulin tolerance, and optimized lipid profiles (total cholesterol, triglycerides, LDL cholesterol), while reducing oil-red staining in hepatic and serum tissues

In diabetic rats, which are known to exhibit severely delayed wound healing, PIC treatment demonstrated elevated glutathione and ascorbic acid levels, improved epithelialization, and enhanced activation of fibroblasts and mast cells in the wound area

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