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The amount of free ENZ in the supernatant was measured using spectrophotometry at a wavelength of 234 nm, using a calibration curve prepared with a standard ENZ solution

The 16S rRNA gene was amplified using the 515f/806r primer pair, which amplifies the V4 region

In addition, senescence-associated secreted factors, such as interleukin 6 (IL-6), interleukin 8 (IL-8), also displayed similar expression profiles, particularly in Cys 2 -starved and erastin-treated cells (Fig