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4x Sterile saline solution ampules (5mL each)

Apply a few drops onto clean skin and gently pat until fully absorbed

When evaluating the differences in the construction of the NALFD/NASH model between the two methods, it was found that the MCD diet could spontaneously lead to liver fibrosis within 24 weeks and significantly affect the expression of genes involved in liver fibrosis pathways

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Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the StepOne Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA) and THUNDERBIRD SYBR qPCR Mix (Toyobo)