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Briefly, HLCs cultured in 12-well tissue culture plates were untreated or treated with omeprazole (OME, 100 M) or phenobarbital (PB, 500 M) for 72 h, with medium replenished every day

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Cells were washed twice with ice-cold RPMI1640 medium with 10% FBS and incubated for 1 h at 4 C, with the indicated concentrations of dimer macrocycles or hHGF in RPMI1640 medium containing 10% FBS

Chronic kidney disease (CKD), particularly in later stages, is a recognized cause of hypercobalaminemia