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doi:10.1002/rmb2.12336

After the cells reached ~ 80% confluence under normal culture conditions, the medium was changed to glucose-free DMEM (Gibco) and the dish was placed in an anaerobic chamber (5% CO2, 95% N2) to induce OGD-related cell injury

Functionally, these findings correlated with reduced iron availability upon dexamethasone pretreatment after 24 h of erastin treatment as assessed by flow cytometry for FerroOrange staining (Fig

It is a signal to slow down and evaluate the delivery method, not necessarily a sign of toxicity