After the lung extracts were centrifuged (12,000 g , 10 min at 4C), the supernatant was collected, and protein concentrations were determined using a Bicinchoninic Acid (BCA) Protein Assay Kit (Beyotime Institute of Biotechnology)
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J Immunol (2004) 173(6):367683
Per the existing literature, palmatine could attenuate LPS-induced inflammation by inhibiting ERK1/2, P38, and Akt/NF-kB signaling pathways (Tang et al., 2021)
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