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The results exhibited a significant reduction in the proliferation activity of MCF-7 cells treated with CuNPs, recorded IC 50 value of 57.2 3.1 g/ml, while nano-chrysin IC 50 was 125.4 6.2 g/ml (IC 50 is the concentration that reduced the initial count of viable MCF-7 cell line by 50% compared to untreated control cells)

They used sterile water for a multi-use protocol