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In an era where marijuana use was deemed illegal, most studies were forced to rely on user-reported data or medical record searches, which typically leaves many unanswered questions as to the frequency and nature of the substance consumed, as well as a wide range of other variables that might affect outcomes

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In line with the increased nicotine concentration in the e-liquids, increasing and high cotinine levels in saliva were observed [17], and high cotinine levels in saliva indicate a potentially high cotinine content in e-liquids sold on the local market

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