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Cells were prepared and stained as per the Leica protocol ( In brief, cells were plated onto coverslips and treated with or without 5FU at the indicated doses for 72 h

13a, Supplementary Table 9), suggesting that the distinct rotamer orientation of H296 ECL2 in Cagri-bound CTR (to accommodate the Glu linker) and/or the observed more open ECL3 conformation induced by the lipidated peptide may facilitate or stabilize an active CTR conformation (Supplementary Fig

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If youre using Trimix, put the medication vial back into the refrigerator