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After thoroughly mixing the liposome solution with the hydrogel precursor, gelation was induced, resulting in liposomes being distributed within the aqueous phase of the hydrogel matrix or adsorbed onto the gel framework

To measure PIP 3 , FoB were stimulated with 5 g/mL anti-IgM (Jackson Immunoresearch 715-006-020) for the indicated time points, then fixed in 2% FA and permeabilized with 0.1% saponin