Nave (non-infected) erythrocytes and Plasmodium falciparum 3D7-infected erythrocytes were cultured at 37C, in low oxygen conditions (1% O 2 , 5% CO 2 , 94% N 2 ), at 4% or 0.4% hematocrit, in incomplete RPMI media (iRPMI: RPMI 1640 HEPES, 50 mg/L hypoxanthine, 10 mg/L gentamicin, pH 6.74), complete RPMI media (cRPMI: incomplete RPMI supplemented with 2.25 g/L sodium bicarbonate and 5 g/L AlbuMAX II), or Ringer solution (1mM CaCl 2 , 5mM glucose, 32 mM HEPES pH 7.4, 5 mM KCl, 1mM MgSO4, 125mM NaCl)
While highly effective, this method is expensive, requires a clinical setting, and involves needles
Each Batch is Individually Tested We ensure that every batch meets rigorous purity and potency standards through third-party testing, confirming the integrity and high quality of our product
208 The platinum-tolerant cell population may exhibit characteristics of cancer stem cells, and platinum-resistance may be related to the FZD7--catenin-Tp63-GPX4 pathway
PMID: 16 DiamantiACapriatiTBizzarriCFerrettiFAncinelliMRomanoFet al