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1 Introduction The annual milk yield of German dairy herds has increased in recent years ( Lipopolysaccharides (LPS) are components of the cell wall of Gram-negative bacteria that act as endotoxins, and are known to stimulate an acute phase response (APR) in mammals ( L-carnitine plays a crucial role in the energy metabolism of cells by acting as a molecular shuttle, facilitating the transport of free long-chain fatty acids mobilized from adipose tissue across the inner mitochondrial membrane ( Previous studies provide evidence that L-carnitine may have beneficial effects on LPS-induced sepsis in rats by reducing serum levels of inflammatory cytokines, disease severity, and lethality ( On the one hand, our aim was to describe the APR in detail in mid-lactating pluriparous dairy cows, when a stable energy status is expected after the transition period
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Raw fluorescence values were corrected for background fluorescence, which was measured in wells without cells containing only medium, one-step assay buffer and test compound
