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Ethical principles of the study complied with the European Directive 010/63/E, the Law on Amendments to the Animal Protection Act (Official Gazette 37/13), the Animal Protection Act (Official Gazette 135/06), the ordinance on the protection of animals used for scientific purposes (Official Gazette 55/13), Federation of European Laboratory Animal Science Associations (FELASA) recommendations, and the recommendations of the Ethics Committee of the School of Medicine, University of Zagreb

The crossover study of an oral methionine load by Bellamy et al (1998) also supports the view that elevated homocysteine levels may promote vascular disease by impairing flow-mediated endothelium dependent vasodilation

These are laboratory research observations only, are not established human or veterinary effects, and no combined effect is claimed

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