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While preclinical evidence is robust, clinical translation remains hindered by challenges such as variable bioavailability, lack of standardized formulations, and insufficient large-scale human trials

(168) reported markedly raised blood TMAO levels in PMW with hip fractures, suggesting that heightened TMAO may be associated with an increased risk of OP and fracture in this population

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These are some examples of how OoC technology can represent a next-generation preclinical testing tool, moving beyond conventional toxicology toward precision immuno-oncology, where patient-derived chips could guide therapeutic decisions and dose optimization while reducing reliance on animal models