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After incubation with primary antibodies followed by horseradish peroxidase-linked secondary antibody, the target protein was visualized by Immobilon Western Chemiluminescent HRP substrates (Millipore)

Nevertheless, IL-22s role in chronic colitis and its association with resistance to ustekinumab treatment underscores its dual nature [94]

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In our research, the effectiveness of PDA@Pt as an anti-inflammatory agent was confirmed through comprehensive real-time fluorescence quantitative analysis (qRT-PCR) and immunofluorescence experiments

There were no significant differences between each group (Figure 4D)